|
KMID : 0364920170420040189
|
|
Journal of Radiation Protection and Research
2017 Volume.42 No. 4 p.189 ~ p.196
|
|
|
Comparison of Skin Injury Induced by β- and γ- irradiation in the Minipig Model
|
|
Kim Joong-Sun
Jang Hyo-Sun
Bae Min-Ji
Shim Se-Hwan
Jang Won-Seok
Lee Sun-Joo
Park Sun-Hoo
Lee Seung-Sook
|
|
|
Abstract
|
|
|
Background: The effects of radiation on tissues vary depending on the radiation type. In this study, a minipig model was used to compare the effects of β-rays from 166Ho and γ-rays from 60Co on the skin.
Materials and Methods: In this study, the detrimental effects of β- and γ-irradiation on the skin were assessed in minipigs. The histopathological changes in the skin from 1 to 12 weeks after exposure to 50 Gy of either β- (using 166Ho patches) or γ- (using 60Co) irradiation were assessed.
Results and Discussion: The skin irradiated by β-rays was shown to exhibit more severe skin injury than that irradiated by γ-rays at 1-3 weeks post-exposure; however, while the skin lesions caused by β-rays recovered after 8 weeks, the γ-irradiated skin lesions were not repaired after this time. The observed histopathological changes corresponded with gross appearance scores. Seven days post-irradiation, apoptotic cells in the basal layer were detected more frequently in β-irradiated skin than in γ-irradiated skin. The basal cell density and skin thickness gradually decreased until 4 weeks after γ- and β- irradiation. In β-irradiated skin lesions, and the density and thickness increased sharply back to control levels by 6-9 weeks. However, this was not the case in γ-irradiated skin lesions. In γ-irradiated skin, cyclooxygenase-2 (COX-2) was shown to be expressed in the epidermis, endothelial cells of vessels, and fibroblasts, while β-irradiated lesions exhibited COX-2 expression that was mostly limited to the epidermis.
Conclusion: In this study, β-rays were shown to induce more severe skin injury than γ-rays; however, the β-rays-induced injury was largely repaired over time, while the γ-rays-induced injury was not repaired and instead progressed to necrosis. These findings reveal the differential effects of γ- and β-irradiation on skin and demonstrate the use of minipigs as a beneficial experimental model for studying irradiation-induced skin damage.
|
|
|
KEYWORD
|
|
|
Minipig, β-irradiation, γ-irradiation, Skin, COX-2
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|